
Postdoctoral Researcher
- Toronto, ON
- Temporary
- Full-time
Number of Vacancies: 1
Site: Princess Margaret Cancer Centre, MaRS Tower
Department: Princess Margaret Research Institute
Reports to: Principal Investigator
Hours: 37.5 hours per week
Shifts: Monday to Friday
Status: Temporary Full-time
Closing Date: September 30, 2025Position Summary:
The Myc oncoprotein is dysregulated in most human cancers and is a key regulator of gene transcription, binding to ~10% of the human genome to drive biological activities associated with the hallmarks of cancer. Successfully targeting MYC would represent a breakthrough in cancer therapy, but due to the intrinsically disordered nature of the MYC protein it has evaded classic drug development approaches for several decades. Our lab has recently had a breakthrough and identified several proteins that interact with MYC. These data provide an unprecedented opportunity to i) better understand MYC regulation and function as a potent cancer driver, and ii) develop anti-MYC activity therapies by blocking critical MYC-protein interactions. This novel line of research has enormous potential to radically advance new knowledge and make a lasting impact to patients and their families.We are searching for a highly motivated Post-Doctoral Researcher with a PhD in a related discipline, such molecular biology or biochemistry. This project will consist of validating and characterizing novel MYC-protein interactions at the cellular and tumor levels, with a focus on proteins that are essential to MYC oncogenic function. The successful candidate will be working at the Princess Margaret Cancer Centre and will benefit from a highly collaborative and interdisciplinary research team.Duties:
- Conduct protein-protein interaction assays, such as proximity ligation assay, co-immunoprecipitation
- Analyze protein-DNA interactions using CUT&RUN or Chromatin Immunoprecipitation followed by sequencing or qPCR
- Perform analyses of gene expression including RT-qPCR, RNA-seq, EU incorporation
- Assay MYC-protein complexes regulating gene transcription and oncogenesis by a variety of methods
- Have strong computational skills to enable genomic analyses and data integration
- Work with databases that enable our research such as Encode and DepMap
- Understand the state-of-the-art at both the methodological and biological levels
- Report results both in writing and orally to individuals as well as collaborative groups
- PhD in molecular biology, biochemistry or related discipline
- Expertise in the area of gene transcription and protein-protein interaction
- Comprehensive experience in using and developing tumor models based in tissue culture and mice
- Work well independently as well as with others as part of a team
- Excellent problem recognition and problem-solving
- Excellent verbal and written communication skills
- Excellent interpersonal skills
- Excellent organizational and time management skills
- Ability to set priorities and work independently with accuracy in a fast-paced multitasking environment
- Competitive offer packages
- Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP
- A flexible work environment
- Opportunities for development and promotions within a large organization
- Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)